The Molecular Biology of Cytoplasmic Capping
Cytoplasmic RNA recapping, the process by which cells can return a 7-methylguanosine (m7G) cap onto an uncapped mRNA, is an epitranscriptomic process with large potential in post-transcriptional gene regulation. Currently, much remains unknown about this potentially vital process. The lab uses both transcriptome-wide (RNA-seq, ribosome profiling, and oxford nanopore long read sequencing) and targeted methods (qPCR, polysome gradients, etc.).